If it's in there, SepsiTest™ will find it!In its concept, SepsiTest™ detection of pathogens is based on validated data using universal rDNA primers. SepsiTest™ allows detection of essentially all eubacterial and fungal pathogens, including both culturable and non-culturable strains. The broad-range primers used with SepsiTest™ bind to conserved regions on rRNA genes and are useful for the screening of pathogens in blood and other samples. Identification of strains is possible by sequencing the amplification product, which contains sequences of highly variable regions for Molzym's online identification tool, SepsiTest BLAST. Thus, SepsiTest™ provides the option of detecting and identifying any bacterial or fungal pathogen in a blood or other clinical sample. More than 345 species have been put to the list of detectable strains by using an alignment algorithm with the primers included for PCR (table 1 shows a selection). Thus, pathogens can be detected in blood and other primary material on a broad basis using SepsiTest™. Sequencing of the amplicon allows direct identification of the pathogen(s) detected. Table 1: Pathogens detectable by SepsiTest™ (selection of strains) Bacteria (Assay Bacteria) | Fungi (Assay Yeasts) | | Gram-negative | Gram-positive | species
| | Genus | # species | genus | # species | genus | # species | Achromobacter
| 1
| Serratia
| 4
| Actinomyces
| 1
| Aspergillus fumigatus | Acinetobacter
| 5
| Shewanella
| 3
| Aerococcus
| 1
| Candida albicans | Actinobacillus
| 2 | Sphingomonas
| 1
| Arthrobacter
| 1
| Candida glabrata | | Aeromonas | 7
| Stenotrophomonas
| 1
| Bacillus
| 20
| Candida kruzeri | Aggregatibacter
| 3
| Vibrio
| 7
| Brevibacterium
| 1
| Candida parapsilosis | Alcaligenes
| 2
| Xanthomonas
| 1
| Clostridium
| 2
| Candida tropicalis | Alteromonas
| 1
| Yersinia
| 2
| Corynebacterium
| 2
| Cryptococcus neoformans | Anaplasma
| 1
| Morganella | 1
| Enterococcus
| 7
| | Bacteroides
| 2
| Neisseria
| 2
| Erysipelothrix
| 1
| | Bifidobacterium
| 9
| Ochrobactrum
| 2
| Exiguobacterium
| 1
| | Brevundimonas
| 2
| Pantoea
| 2
| Geobacillus
| 2
| | Burkholderia
| 6
| Pasteurella
| 1
| Frankia
| 1
| | Butyrivibrio
| 1
| Photobacterium
| 1
| Kocuria
| 1
| | | Campylobacter | 2
| Plesiomonas
| 1
| Lactobacillus
| 28
| | | Chlamydophila | 2
| Proteus
| 2
| Lactococcus
| 3
| | | Citrobacter | 1
| Pseudomonas
| 18
| Leifsonia
| 1
| | | Comamonas | 1
| Rahnella
| 1
| Leuconostoc
| 2
| | | Delftia | 1
| Ralstonia
| 3
| Microbacterium
| 2
| | | Ehrlichia | 2
| Riemerella
| 1
| Micrococcus
| 1
| | | Enterobacter | 6
| Salmonella
| 2
| Mycobacterium
| 10
| | | Escherichia | 1
| | | Nocardia
| 3
| | | Flavobacterium | 3
| | | Paenibacillus
| 3
| | | Haemophilus | 4
| | | Pediococcus
| 2
| | Hafnia
| 1
| | | Propionibacterium
| 1
| | Helicobacter
| 1
| | | Rhodococcus
| 3
| | Klebsiella
| 2
| | | Staphylococcus
| 19
| | Leptospira
| 5
| | | Streptococcus
| 6
| | | Megashaera | 1
| | | Streptomyces
| 2
| | Methylobacterium
| 1
| | | Weissella
| 2
| | Moraxella
| 2
| | | | | | | | | Sum:
| 97
| Sum:
| 129
| |
|
Technology 
