Latest publication:

Staphylococcus aureus and mecA detection in whole blood using pre-analytical tool MolYsis and Real-Time PCR

In a prospective study, Wellinghausen et al. investigated 902 blood samples from 384 patients for Staphylococcus aureus bacteraemia and the presence of methicillin resistance gene, mecA. For this purpose, the authors used the pre-analytical tool, MolYsis, for the extraction of DNA from up to 5 ml blood samples from critically ill patients and Real-Time PCR assays. The authors discuss the promissing results with regard to the usefulness of the molecular test for S. aureus/mecA diagnosis. >>read more

References & Publications on SepsiTest™

·   PCR Detection of Sepsis-Inducing Pathogens in Blood Using SepsiTest™: In a study including 134 blood samples from 113 critically ill patients, a team of researchers from University Clinic Ulm, Clinic Cologne, and Molzym, Bremen, compared two complete systems of directed bacteria DNA extraction from blood samples and broad-spectrum real-time PCR (RT PCR) detection of pathogens among each other and to blood culture (BC) results. It came out that 1 ml of blood sample (SepsiTest™) was as sensitive in detecting pathogens as 5 ml blood sample (MolYsis Complete5 extraction plus Mastermix 16S assay). The study further showed that RT-PCR proved to be highly sensitive (89.3 %) in detecting bacteremia. Sequencing of amplicons of PCR positive samples confirmed the result, and BLASTN homology search identified 17 species, including less frequently occuring pathogens like Haemophilus sp., Gemella sp. and Proteus vulgaris. Among the RT-PCR positive, BC negative samples, 6 were confirmed by positive swabs taken at other sites. Most RT-PCR samples contained multiple strains. The Poster was presented at the meeting of the German Society of Hygiene and Microbiology (DGHM) in Dresden, Germany (September 21-24). To download the poster, please click >> here.

 ·   H. Mühl et al. (2008) Activity and DNA contamination of commercial polymerase chain reaction reagents for the universal 16S rDNA real-time polymerase chain reaction detection of bacterial pathogens in blood. Diagnostic Microbiology and Infectious Disease, 2008 Aug 20. (Epub ahead of print)

·   C. Disqué et al. (2008) PCR Detection of Sepsis-Inducing Pathogens in Blood Using SepsiTest™, Poster at DGHM-Meeting 2008

·   S. Gebert et al. (2008) Rapid detection of pathogens in blood culture bottles by real-time PCR in conjunction with the pre-analytic tool MolYsis. J. Infec., in press

·   M. Handschur et al. (2008) Preanalytic removal of human DNA eliminates false signals in general 16S rDNA PCR monitoring of bacterial pathogens in blood, Comp Immunol Microbiol Infect Dis. 2008 Feb 6., in press

·   C. Disqué (2007) Einfluss der DNA-Extraktionsmethode auf die quantitative PCR von Sepsis-Erregern. Biospektrum 6/2007: 627- 629

·   S. Gebert et al. (2007) Rapid detection of pathogenic bacteria in blood samples by eubacterial Gram-differentiating multiplex real-time PCR in conjunction with pre-analytic DNA preparation kit MolYsis. Poster at DGHM-Meeting 2007

·   W. Hansen et al. (2007) Evaluation of MolYsis blood DNA removal kit as pre-analytical tool for application to 16S rRNA PCR in molecular pathogen diagnostics. Poster at 2007 NVMM-Meeting in Scheveningen.

·   H.P. Horz et al. (2008)   Selective isolation of bacterial DNA from human clinical specimens. J. Microbiol. Methods 72: 98-102

·   H. Mühl + U. Fischer (2008) DNA Contamination in Commercially Available PCR Reagents: A Comparative Study Poster at VAAM 2008, Frankfurt